Levels of major selenoproteins in T cells decrease during HIV infection and low molecular mass selenium compounds increase

Vadim N. Gladyshev; Thressa C. Stadtman; Dolph L. Hatfield; Kuan-Teh Jeang

doi:10.1073/pnas.96.3.835

Figure 2

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SDS/PAGE analysis of T cell Se-proteins separated by DEAE-Sepharose chromatography. ⁷⁵Se-labeled T cells were sonicated and centrifuged, and soluble selenoproteins were chromatographed on a DEAE-Sepharose column (see Materials and Methods). The peak radioactive fractions were analyzed by SDS/PAGE. Lane 1, sonic extract; lane 2, sedimented pellet; lane 3, supernatant fraction; lane 4, proteins not retained on DEAE column (flow through fraction); lane 5, 26-kDa protein (eluted with 150 mM NaCl); lane 6, 21-kDa protein (eluted with 200 mM NaCl); lane 7, 57-kDa protein (eluted with 250 mM NaCl); lane 8, 15-kDa protein (eluted with 350 mM NaCl); lane 9, mass standards. (A) Coomassie blue-stained gel. (B) PhosphorImager detection of ⁷⁵Se-labeled proteins in lanes 1–8 of A.