Figure 3

PIAS1 and PIAS3 bind to the second CxxC motif and adjacent region of MBD1. (A) Schematic representation of human PIAS1 protein and its known domains. The regions conserved between PIAS1 and PIAS3 (>80% homology) are indicated. (B) MBD1 deletion constructs were used to identify regions of MBD1 that bind PIAS1 and PIAS3 in yeast two-hybrid assays. The interactions were scored by growth on -Leu/-Trp/-His/-Ade (QDO) medium. (C–D) GST-MBD1 deletion proteins were used to pull down in vitro translated HA-tagged PIAS1 and PIAS3 proteins.