Fig. 2.

Activation of Akt and phosphorylation of GSK3. (A) Protein lysates from dorsolateral (DLP) and VP of MPAKT (Tg) and wild-type (Wt) littermate mice at 15, 35, 47, and 60 wk were immunoblotted with anti-pS473 (Upper) and pan-Akt (Lower) antisera. The positions of endogenous murine Akt (filled arrow) and myr-HA-Akt-1 (open arrow) are indicated. (B) Protein lysates from VP, DP, and LP from MPAKT and wild-type mice were immunoblotted with anti-pS473 (Top), anti-phospho-GSK3 (pS9; Middle), and anti-pan GSK3 (Bottom). (C–H) IHC staining of tissue sections from VP or LP from wild-type (Wt) and MPAKT (Tg-Akt) with anti-pS473 or anti-phospho GSK-3 (pS9) antisera as indicated (C–H, ×200; Insets C and D, ×400).