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FIG. 3.

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Cleavage of endogenous Rac GTPases by recombinant caspase 3 in vitro. Whole-cell extracts from healthy JLP-119 cells containing endogenous Rac GTPases were incubated for different times at 37°C with purified recombinant caspase 3 or 8 at final concentrations of 5 ng/μl in a caspase reaction buffer containing 50 mM HEPES (pH 7.5), 100 mM NaCl, 1 mM EDTA, 10% (wt/vol) sucrose, and 10 mM dithiothreitol. Proteins were examined by Western blot immunoassay with the indicated antibodies.

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