Identification of a regulator of transcription elongation as an accessory factor for the human Mediator coactivator -- Malik et al. 104 (15): 6182 Data Supplement - HTML Page - index.htslp -- Proceedings of the National Academy of Sciences

Malik et al. 10.1073/pnas.0608717104.

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Fig. 6. Immunoblot analysis of Mediator preparations. Mediator was affinity purified (aFLAG IP) over anti-FLAG M2-agarose (using 150 mM KCl in the binding and washing buffer) from nuclear extract (NE) of HeLa cells that stably express the FLAG-tagged MED10/NUT2 (f:MED10/NUT2) subunit (lane 4). Standard HeLa nuclear extract served as control (lane 3). Antibodies against the indicated Mediator subunits and against hSpt5 (Bottom) were used in the immunoblot. Inputs from the starting extracts were also analyzed (lanes 1 and 2).