Figure 1

TAF_IIs are required for MMS-induced chromatin remodeling of RNR3. (A) Nucleosome mapping in TAF_II mutants. Wild type (SW87) and strains containing temperature-sensitive mutations in yeast taf1-2 (YSW93) and taf12-9 (YJR21-9) were grown and treated with MMS at 37°C as described in the text. Cells were then harvested to prepare nuclei for chromatin mapping and RNA for Northern blotting. The nucleosome organization of the RNR3 promoter is illustrated on the left side of the panel and is based on previous detailed mapping studies (Li and Reese 2001). (B) Northern blot analysis of RNR3 mRNA levels in repressed and MMS-treated cells. scR1 is a loading control. Quantification of Northern blots was performed using the ImageQuant software, and was normalized to the signal in the −MMS sample of the wild type, which was arbitrarily defined as a unit of 1.