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Figure S2. Normal DNA methylation of subtelomeric domains in Suv39h-deficient ES cells. (A) Scheme of chromosome 1 depicting the subtelomeric region used for bisulfite genomic sequencing. Eight independent clones per genotype were analyzed. Yellow and blue represent the frequency of methylated and unmethylated CpG dinucleotides, respectively, at each position. Quantification of the percentage of methylated CpGs per sample after bisulfite genomic sequencing of eight clones of each genotype is shown in the bottom panel. Primer sequences are provided in Materials and methods. (B) Scheme of chromosome 2 depicting the subtelomeric region used for bisulfite genomic sequencing. Eight independent clones per genotype were analyzed. Yellow and blue represent the frequency of methylated and unmethylated CpG dinucleotides, respectively, at each position. Quantification of the percentage of methylated CpGs per sample after bisulfite genomic sequencing of eight clones of each genotype is shown in the bottom panel. Primer sequences are provided in Materials and methods. (A and B) Error bars correspond to the total number of clones analyzed (n).