siRNA-Mediated β-Catenin Knockdown in Human Hepatoma Cells Results in Decreased Growth and Survival

Gang Zeng; Udayan Apte; Benjamin Cieply; Sucha Singh; Satdarshan P S Monga

doi:10.1593/neo.07469

Figure 2

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Changes in β-catenin expression and localization after siRNA treatment in Hep3B and HepG2 cells by immunofluorescence. Left panel: Original magnification, ×600. Right panel: Original magnification, ×1000. (A) Membranous and nuclear (arrowhead) β-catenin is observed in Hep3B cells at 48 hours after control siRNA transfection. (B) Decreased expression and nuclear localization of β-catenin at 48 hours after β-CTNNB1 siRNA transfection in Hep3B cells. (C) Membranous and nuclear (arrowhead) β-catenin is observed in HepG2 cells at 48 hours after control siRNA transfection. (D) Decreased expression and nuclear localization of β-catenin at 48 hours after β-CTNNB1 siRNA transfection in HepG2 cells. A small subset of cells continued to show nuclear β-catenin (arrowheads).