FIGURE 5

Effect of substrate stiffness of F-actin organization. (A) Western blot for actin from pellet formed by low-speed (15,000 × g) centrifugation of fibroblast lysates. Cells were lysed 24 h after plating on fibronectin-laminated PA gels with 0.7 kPa, 4.5 kPa, and 15.2 kPa elastic moduli. (B) Densitometric quantification of Western blots for actin sedimenting at low speed from fibroblasts plated on PA gels of 0.7 kPa, 4.5 kPa, 15.2 kPa, or tissue culture plastic. All values normalized to total protein in cell lysate. Error bars are representative standard deviations from three repeats.