Figure 2.

Induction of CCR6 on Th17 cells in vitro. (A) Retroviral vector, pMxs-RORγt-IRES-GFP (RORγt-IG), or control pMxs-IRES-GFP (Control-IG), was transduced into BALB/c CCR6⁻CD4⁺ T cells, as previously described (reference 7), and the cells expressing GFP were stained for CCR6 on day 4 after transduction. (B) The mean percentages ± SD of CCR6⁺ or IL-17⁺ cells among CD4⁺GFP^high cells (as gated in A) in three independent experiments are shown. (C) CD4⁺CD25⁻CCR6⁻ T cells were in vitro driven to differentiate to Th17 cells in the indicated conditions, as previously described (reference 21). CCR6 and intracellular IL-17 gated on CD4⁺ T cells were stained on day 5. Results in A and C represent three independent experiments, and the percentage of cells in each quadrant is shown.