Figure 4.

The apoptotic program represents a selective advantage for yeast cultures. (A) Survival of wild-type and yca1 null mutant strain during exponential growth (6 h after inoculation) in dependency of the age of the preculture. Data represent mean ± SEM. (B) Competition assay between wild-type and yca1 null mutant strain. Equal amounts of cells of exponentially growing cultures of wild-type and yca1 null mutant strains were mixed, and the percentage of living cells of both strains was monitored by plating assay (100% is the total number of living cells). Experiments were performed four times with similar results. (C) Survival of wild-type and yca1 null mutant strain after treatment with H₂O₂ at low cell density. Aged or overnight precultures were inoculated at 3 × 10⁵ cells/ml in fresh medium, grown for 3 h, treated with H₂O₂, and analyzed for survival after 3 h. Data represent mean ± SEM.