Figure 1.

Visualizing lipid rafts using electron microscopy and spatial point pattern analysis. (a) Anti-GFP labeling is specific, ending at the edge of a typical GFP-tH sheet. (b) 60 min of 1% cyclodextrin treatment depletes cell surface cholesterol, detected by filipin labeling (blue). (c) Pooled K-function analyses of the spatial distributions of GFP-tH; L(r) − r values above the 99% confidence interval for CSR (99% CI; closed circles) indicate clustering at that value of r. Untreated GFP-tH (t = 0, red line) shows maximal deflection from CSR at r = 22 nm. Cyclodextrin-treated cells show a time-dependent loss of GFP-tH clustering such that at t = 60 min, GFP-tH is not clustered. K-functions are means (n ≥ 9 for each condition) standardized on the 99% CI. Bar, 100 nm.