Figure 6

A subset of CD1d1–αGalCer tetramer⁺ cells are NK1.1⁻ and express upregulated levels of CD49d. Liver lymphocytes from normal C57BL/6 mice were stained with tetramer-Cychrome, NK1.1-PE, and anti-Vβ–FITC or CD49d-FITC. Note that most tetramer⁺ NK1.1⁺ cells were CD49d⁻ whereas tetramer⁺ NK1.1⁻ cells were CD49d⁺. Both subsets exhibited the same bias in Vβ8 (middle) and Vβ7 and Vβ2 (not shown). Similar results were found for splenocytes and in Vα14-Jα281 TCR α chain transgenic mice.