Figure 5

N-cad expression in the anterior neural keel is altered in MZoep mutants. (A-F) N-cad immunostaining of tissue sections from WT and MZoep embryos. (A, B) Sagittal sections though the anterior neural keel with anterior to the left and dorsal to the top. (C-D') Parasagittal sections with anterior to the left and dorsal to the top. Panels C' and D' are higher magnifications of the boxed regions in panels C and D, respectively. Arrows point to N-cad labeling in the cell membrane, while the arrowhead indicates labeling in the cytoplasm. (E, F) Transverse sections through the anterior neural tube, dorsal to the top. n ≥ 30 embryos for each sample. Embryos are at 95–100% epiboly (95–100%) or the 5–6 somite stage (5–6 s). Scale bars: 40 μm (C, D, E, F), 20 μm (A, B, C', D').