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J Virol. 1994 Jan; 68(1): 535–540.
doi: 10.1128/jvi.68.1.535-540.1994
PMCID: PMC236318
PMID: 8254768

Restriction and enhancement of human immunodeficiency virus type 1 replication by modulation of intracellular deoxynucleoside triphosphate pools.

A Meyerhans, J P Vartanian, C Hultgren, U Plikat, A Karlsson, L Wang, S Eriksson, and S Wain-Hobson
Author information Copyright and License information PMC Disclaimer

Abstract

Human immunodeficiency virus type 1 (HIV-1) replication is shown to be sensitive to the intracellular concentration of deoxynucleoside triphosphate substrates. Addition of thymidine to established cell lines resulted in a dramatic reduction of virus production. The effect could be substantially alleviated by addition of deoxycytidine, which, alone, enhanced viral titers by a factor of 2 to 3. Hydroxyurea treatment abolished HIV-1 replication in peripheral blood mononuclear cells and could be reversed by deoxyadenosine. These data show that HIV-1 replication occurs under suboptimal DNA precursor conditions.

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Selected References

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