FIGURE 9

IFN-γ and TNF-α signal through a cytokine relay network involving the type I interferon receptor. Wild-type 2fTGH cells and hIFNAR2-deficient U5A cells were infected for 16 hours with VSV-GFP. GFP expression was analyzed by flow cytometry. Data are expressed as mean +/− SEM of three replicates. A, 2fTGH and U5A cells were infected in the absence (open bars) or presence of 100 U/ml rhIFN-αA (hatched bars), 2.5 ng/ml rhIFN-γ (black bars), 2.5 ng/ml rhTNF-α (double hatched bars), or a combination of 2.5 ng/ml rhIFN-γ and 2.5 ng/ml rhTNF-α (gray bars). B, 2fTGH and U5A cells were infected in the absence (black bars) or presence of 100 U/ml rhIFN-αA (hatched bars) or 10% (v/v) T cell conditioned media generated from IL-12 + IFN-α activated hCD4⁺ T cells (white bars). *, p < 0.05, one-way ANOVA.