Figure 3

Primer rescue by HIV-1 RT. 5′-³²P-labeled, ddAMP-terminated L32 primer annealed to WL50 template was incubated with excess HIV-1 RT and the indicated concentrations of PPase-treated ATP (lanes 1–8) or GTP (lanes 9–16) at 37°C for 10 min. After heat inactivation of the RT, the primer was extended by incubation with exonuclease-free Klenow polymerase and dNTPs for 10 min at 37°C. The products were separated by electrophoresis through a 20% denaturing polyacrylamide gel. The original L32-ddAMP primer is indicated as primer and extended primer products are indicated as ext. primer.