Stem/progenitor cells from bone marrow decrease neuronal death in global ischemia by modulation of inflammatory/immune responses

Hirokazu Ohtaki; Joni H. Ylostalo; Jessica E. Foraker; Andrew P. Robinson; Roxanne L. Reger; Seiji Shioda; Darwin J. Prockop

doi:10.1073/pnas.0803670105

Fig. 2.

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Microarray assays of hippocampi 2 days after tCCAO and 1 day after injection of HBSS or hMSCs. (A) Effects of ischemia on the mouse transcriptome. Signal intensities (SI) were compared between samples from uninjured/HBSS and ischemia/HBSS mice. (B) Venn diagram of 586 mouse genes up-regulated by ischemia, eight genes further up-regulated by injection of hMSCs, and 80 genes down-regulated by the hMSCs. (C) Venn diagram of 41 mouse genes down-regulated by ischemia and one of the down-regulated genes up-regulated by injection of hMSCs. (D) Effects of hMSCs on the transcriptome of mice after tCCAO. The hMSCs produced up-regulation of only three mouse genes (Lyz, lysozyme; Ym1, also known as Chi313; and Tgbi, TGFβ induced protein). The hMSCs produced down-regulation of 80 mouse genes. The most common GO terms are indicated. (E) Venn diagram of 189 human genes (170 nonredundant) up-regulated in hMSCs by the ischemic environment. (F) Venn diagram of 57 human genes (54 non-cross-hybridizing) down-regulated by the ischemic environment. The genes were filtered for cross-hybridization as indicated in SI Methods. (G) Effects of the ischemic microenvironment on the hMSC transcriptome.