Substrate Modulus Directs Neural Stem Cell Behavior

Krishanu Saha; Albert J. Keung; Elizabeth F. Irwin; Yang Li; Lauren Little; David V. Schaffer; Kevin E. Healy

doi:10.1529/biophysj.108.132217

FIGURE 6

An external file that holds a picture, illustration, etc.
Object name is BIO.132217.wc.f6.jpg

The proportion of neurons versus glia under mixed differentiation is a strong function of modulus. (A) Phase-contrast images of aNSCs differentiated under mixed glial and neuronal differentiation conditions (1 μM retinoic acid and 1 v/v % fetal bovine serum). (B) Immunostaining of aNSCs differentiated under mixed differentiation conditions. Cell-lineage markers: progenitor cell marker nestin (green), mature neuronal marker β-tubulin III (red), and astrocytic marker GFAP (blue). (C) Percentage of cells that stained positive for β-tubulin III (red) is on left axis, and for GFAP (blue), on right axis. Error bars are 95% confidence intervals. Means with same group letter are not significantly different from each other (analysis of variation Tukey-Kramer test, p < 0.05). Note the log x-axis. All images were taken after 6 days of culture.