Table 1
SSCP Amplimers and Conditions Used to Screen Retinal Degeneration Cohort
| Exon | Primer Sequences 5′-3′ | Amplimer Size | Restriction Enzyme |
|---|---|---|---|
| 1 | GGCGCGGCGTCAGCAGTAGCAG | 367 | AluI |
| TCGCCGTGCCACGTCCGTCTGCTC | |||
| 2 | ACCCCAGTAGACCTTTCGCT | 323 | NlaIV |
| ATGCCCTGCCCCTGAGCAAG | |||
| 3 | TGGGTGATAAACTCTTTAGCTGG | 255 | |
| GGAAGTGTGGTCAGAGCCG | |||
| 4 | CCGGCTCTGACCACACTT | 163 | |
| GCCTCTGAGGTGGGGACT | |||
| 5 | GCTTTCTTCCAGCCTGTTCCT | 281 | |
| ACACCCAGCCCTGCTTCCC | |||
| 6 | CCTTCTCTCTCACCTGCCAAC | 202 | |
| AACAACGGGACTGTGGAC | |||
| 7 | AGTGGAATCTCTGGAGTGGTC | 378 | |
| CCTGGGTCCTCATAAACCTC | |||
| 8 & 9 | TTCATCCACTCAGGCTCTCC | 560 | Cac81 |
| CTGGGGAACAAAGGCGAGG | |||
| 10 & 11 | ACACTCATCCTGGTGGTATTTG | 643 | Eco01091 |
| CCATCTGGGGAAGTCGGTG | |||
| 12 | AAGAGGTGGGGTGGGGT | 267 | BamHI |
| CAAGGGTGGAGAAGAGCG | |||
| 13 | GCTTCCTTTCTCTCGCTCTTC | 202 | |
| ACCTCGCCAACCCACTGC | |||
| 14 | GGGAAAGGGTTCTGGGAAG | 153 | |
| TGTGCCCAAAAGTGGACAC |