Fewer Latent Herpes Simplex Virus Type 1 and Cytotoxic T Cells Occur in the Ophthalmic Division than in the Maxillary and Mandibular Divisions of the Human Trigeminal Ganglion and Nerve

Katharina Hüfner; Anja Horn; Tobias Derfuss; Christine Glon; Inga Sinicina; Viktor Arbusow; Michael Strupp; Thomas Brandt; Diethilde Theil

doi:10.1128/JVI.02464-08

FIG. 2.

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Di-I tracing of the maxillary and mandibular branches of the human TG. Human TG showing the distribution of Di-I-labeled neurons (red dots) in comparison to unlabeled neurons (gray dots) after Di-I exposure to the ophthalmic (A) or the maxillary (B) branch. Only a very small number of Di-I-labeled neurons could also be found in the adjacent divisions of the TG as defined by the traversing fiber bundles of the TG nerve branches. This was not due to tracer contamination of the neighboring nerve branches (A and B, arrowheads). (C) Photograph of Di-I-labeled nerve fibers (arrows) in the ophthalmic nerve and the tracer-labeled neurons in the ophthalmic subdivision. (D) High-power magnification of TG neurons demonstrating the Di-I labeling, which fills the whole cell body homogeneously, whereas Di-I-negative neurons (arrows) exhibited only an unspecific fluorescence signal confined to lipofuscin granules (E). Scale bars: A and B, 5 mm; C, 300 μm; D and E, 50 μm.