Learn more: PMC Disclaimer | PMC Copyright Notice
Microplate technique to determine hemolytic activity for routine typing of Listeria strains.
Abstract
Because the hemolysis produced by Listeria monocytogenes and Listeria seeligeri on blood agar is frequently difficult to interpret, we developed a microplate technique for the routine determination of hemolytic activity with erythrocyte suspensions. This microtechnique is a simple and reliable test for distinguishing clearly between hemolytic and nonhemolytic strains and could be used instead of the CAMP (Christie-Atkins-Munch-Petersen) test with Staphylococcus aureus in the routine typing of Listeria strains. Furthermore, our results suggest that the quantitation of the hemolytic activity of the Listeria strains, along with the D-xylose, L-rhamnose, and alpha-methyl-D-mannoside acidification tests, allows the differentiation of L. monocytogenes, L. seeligeri, and Listeria ivanovii. We also observed that the treatment of erythrocytes with crude exosubstances of rhodococcus equi, Pseudomonas fluorescens, Acinetobacter calcoaceticus, and S. aureus enhanced the hemolytic activity of all Listeria strains with this characteristic.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1019K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bernheimer AW, Avigad LS. Mechanism of hemolysis by Renalin, a CAMP-like protein from Corynebacterium renale. Infect Immun. 1982 Jun;36(3):1253–1256. [PMC free article] [PubMed] [Google Scholar]
- Bernheimer AW, Linder R, Avigad LS. Stepwise degradation of membrane sphingomyelin by corynebacterial phospholipases. Infect Immun. 1980 Jul;29(1):123–131. [PMC free article] [PubMed] [Google Scholar]
- Domínguez Rodriguez L, Fernández Garayzabal JF, Vazquez Boland JA, Rodriguez Ferri E, Suarez Fernández G. Isolation de micro-organismes du genre Listeria à partir de lait cru destiné à la consommation humaine. Can J Microbiol. 1985 Oct;31(10):938–941. [PubMed] [Google Scholar]
- Dominguez Rodriguez L, Suárez Fernández G, Fernández Garayzabal JF, Rodriguez Ferri E. New methodology for the isolation of Listeria microorganisms from heavily contaminated environments. Appl Environ Microbiol. 1984 May;47(5):1188–1190. [PMC free article] [PubMed] [Google Scholar]
- Groves RD, Welshimer HJ. Separation of pathogenic from apathogenic Listeria monocytogenes by three in vitro reactions. J Clin Microbiol. 1977 Jun;5(6):559–563. [PMC free article] [PubMed] [Google Scholar]
- Khan MA, Seaman A, Woodbine M. Proceedings: Listeria monocytogenes-haemolysin: lecithinase. Acta Microbiol Acad Sci Hung. 1972;19(4):341–352. [PubMed] [Google Scholar]
- Nakazawa M, Nemoto H. Synergistic hemolysis phenomenon of Listeria monocytogenes and Corynebacterium equi. Nihon Juigaku Zasshi. 1980 Oct;42(5):603–607. [PubMed] [Google Scholar]
- Pongratz G, Seeliger HP. Hämolysewirkungen durch Listeria innocua auf Schaferythrozyten. Zentralbl Bakteriol Mikrobiol Hyg A. 1984 Aug;257(3):296–307. [PubMed] [Google Scholar]
- Rocourt J, Alonso JM, Seeliger HP. Virulence comparée des cinq groupes génomiques de Listeria monocytogenes (sensu lato). Ann Microbiol (Paris) 1983 May-Jun;134A(3):359–364. [PubMed] [Google Scholar]
- Rocourt J, Schrettenbrunner A, Seeliger HP. Différenciation biochimique des groupes génomiques de Listeria monocytogenes (sensu lato). Ann Microbiol (Paris) 1983 Jan-Feb;134A(1):65–71. [PubMed] [Google Scholar]
- Seeliger HP. Apathogene listerien: L. innocua sp. n. (Seeliger et Schoofs, 1977). Zentralbl Bakteriol Mikrobiol Hyg A. 1981;249(4):487–493. [PubMed] [Google Scholar]
- Seeliger HP, Schrettenbrunner A, Pongratz G, Hof H. Zur Sonderstellung stark hämolysierender Stämme der Gattung Listeria. Zentralbl Bakteriol Mikrobiol Hyg A. 1982 Jun;252(2):176–190. [PubMed] [Google Scholar]
- Skalka B, Smola J. Selective diagnostic medium for pathogenic Listeria spp. J Clin Microbiol. 1983 Dec;18(6):1432–1433. [PMC free article] [PubMed] [Google Scholar]
- Skalka B, Smola J, Elischerová K. Routine test for in vitro differentiation of pathogenic and apathogenic Listeria monocytogenes strains. J Clin Microbiol. 1982 Mar;15(3):503–507. [PMC free article] [PubMed] [Google Scholar]
- Skalka B, Smola J, Elischerová K. Different haemolytic activities of Listeria monocytogenes strains determined on erythrocytes of various sources and exploiting the synergism of equi-factor. Zentralbl Veterinarmed B. 1982 Sep;29(8):642–649. [PubMed] [Google Scholar]
- Skalka B, Smola J, Elischerová K. Hemolytic phenomenons under the cultivation of Listeria innocua. Zentralbl Bakteriol Mikrobiol Hyg A. 1983 Feb;253(4):559–565. [PubMed] [Google Scholar]
- Weis J, Seeliger HP. Incidence of Listeria monocytogenes in nature. Appl Microbiol. 1975 Jul;30(1):29–32. [PMC free article] [PubMed] [Google Scholar]