TABLE 2.
Primers
| Function and primer | Sequence (5′→3′) | Site createda |
|---|---|---|
| Probe synthesis | ||
| LPD1 | ACCGACACTGATCCACTCAT | |
| LPD2 | CACCGATCTCGTCATCAAACG | |
| LPD3 | CACCGATCTCGTCATCAAACG | |
| LPD4 | GTCTAATATGTCGTTTTAATC | |
| LPD5 | GACTGCAGGGCGACCGTTTTCCCGCAAGC | PstI |
| LPU1 | AATGACGTCATCCACATCAC | |
| LPU2 | TCAGAACGTGCGTTTCGATA | |
| LPU3 | GTCGAAGATCCAAGCTAAAC | |
| Deletion of usp1 | ||
| LPDELU1 | GATAAGCTTTATAGCCATCCTTGCT | HindIII |
| LPDELU2 | GTTCTGCAGTTCTAAGATTGCTGG | PstI |
| LPDELU3 | TTGCTGCAGGATAGACAAGTGGCT | PstI |
| LPDELU4 | AACGGATCCAGTTAGCACACTTAC | BamHI |
| Plasmid construction | ||
| LPDLOC1 | AATGAACAATAGCAGTCAAAACAA | |
| LPDLOC2 | ACGTTTGTCCTACCACCACATTT | |
| LPD6 | GGAGCATGCTAATGGTTGCTGGTTTA | SphI |
| LPD7 | GGAGCATGCACGTTTGTCCTACCAC | SphI |
| LPU4 | AAGCCATGGAAAATCAAAAAATGC | NcoI |
| LPU5 | AATCTCGAGTTACCGAACAACGAT | XhoI |
| LPR1 | CCCATGGCGCAAAAAAATAAGTTACAA | NcoI |
aThe sites are underlined in column 2.