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Plaque assay of neonatal calf diarrhea virus and the neutralizing antibody in human sera.
Abstract
Neonatal calf diarrhea virus (a bovine rotavirus) formed distinct plaques in monolayers of MA-104 cells, an established macacus rhesus monkey kidney cell line, when diethylaminoethyl dextran and trypsin were included in the overlay medium. By using this plaque assay method, titration of neutralizing antibody to neonatal calf diarrhea virus was made feasible. It was demonstrated that some human sera contained neutralizing antibody to this agent.
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- Bishop RF, Davidson GP, Holmes IH, Ruck BJ. Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis. Lancet. 1973 Dec 8;2(7841):1281–1283. [PubMed] [Google Scholar]
- Fernelius AL, Ritchie AE, Classick LG, Norman JO, Mebus CA. Cell culture adaptation and propagation of a reovirus-like agent of calf diarrhea from a field outbreak in Nebraska. Arch Gesamte Virusforsch. 1972;37(1):114–130. [PubMed] [Google Scholar]
- Flewett TH, Bryden AS, Davies H. Letter: Virus particles in gastroenteritis. Lancet. 1973 Dec 29;2(7844):1497–1497. [PubMed] [Google Scholar]
- Flewett TH, Bryden AS, Davies H, Woode GN, Bridger JC, Derrick JM. Relation between viruses from acute gastroenteritis of children and newborn calves. Lancet. 1974 Jul 13;2(7872):61–63. [PubMed] [Google Scholar]
- Hara M, Mukoyama J, Tsurahara T, Saito Y, Tagaya I. Letter: Duovirus in schoolchildren with gastroenteritis. Lancet. 1976 Feb 7;1(7954):311–311. [PubMed] [Google Scholar]
- Kapikian AZ, Cline WL, Mebus CA, Wyatt RG, Kalica AR, James HD, Jr, VanKirk D, Chanock RM. New complement-fixation test for the human reovirus-like agent of infantile gastroenteritis. Nebraska calf diarrhea virus used as antigen. Lancet. 1975 May 10;1(7915):1056–1061. [PubMed] [Google Scholar]
- Kapikian AZ, Kim HW, Wyatt RG, Cline WL, Arrobio JO, Brandt CD, Rodriguez WJ, Sack DA, Chanock RM, Parrott RH. Human reovirus-like agent as the major pathogen associated with "winter" gastroenteritis in hospitalized infants and young children. N Engl J Med. 1976 Apr 29;294(18):965–972. [PubMed] [Google Scholar]
- Kapikian AZ, Kim HW, Wyatt RG, Rodriguez WJ, Ross S, Cline WL, Parrott RH, Chanock RM. Reoviruslike agent in stools: association with infantile diarrhea and development of serologic tests. Science. 1974 Sep 20;185(4156):1049–1053. [PubMed] [Google Scholar]
- Konno T, Suzuki H, Ischida N. Letter: Reovirus-like agent in Japanese infants with gastroenteritis. Lancet. 1975 Apr 19;1(7912):918–919. [PubMed] [Google Scholar]
- Mebus CA, Kono M, Underdahl NR, Twiehaus MJ. Cell culture propagation of neonatal calf diarrhea (scours) virus. Can Vet J. 1971 Mar;12(3):69–72. [PMC free article] [PubMed] [Google Scholar]
- Welch AB, Twiehaus MJ. Cell culture studies of a neonatal calf diarrhea virus. Can J Comp Med. 1973 Jul;37(3):287–294. [PMC free article] [PubMed] [Google Scholar]