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Methods. Author manuscript; available in PMC 2010 Nov 1.
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Table 2
Cell lines successfully used in the retrotransposition assay
| Cell Line (organism) | Description | Reference |
|---|---|---|
| 143B TK-(human) | Osteosarcoma | [56] |
| 293T (human) | Embryonic kidney with large T antigen | [36] |
| CC-2509 (human) | Primary fibroblasts | [36] |
| CC-2591 (human) | Primary hepatocytes | [36] |
| CHO-UV20 (hamster) | Defective in DNA repair (ERCC1-) | [54] |
| CRL-12203 (chicken) | Embryonic fibroblasts | [101] |
| DLD-1 (human) | P53 defective | [51] |
| Gli36 (human) | Glioma | [36] |
| H1, H7, H9, BG01,HSF-6, Cyth25 (human) | Human embryonic stem cells | [102] |
| H460 (human) | Lung adenocarcinoma | [103] |
| HCT116 (human) | Colon cancer | [31] |
| HEK293 (human) | Embryonic kidney | [44] |
| Hep3B (human) | Hepatocellular carcinoma | [36] |
| HT1080 (human) | Fibrosarcoma, both p53 wild-type and defective | [51] |
| IMR-90 (human) | Primary fibroblasts, only work when dividing | [104] |
| LTK- (mouse) | L cells lacking thymidine kinase | [7] |
| MCF-7 (human) | Breast adenocarcinoma | [51] |
| MCF-7-bcl2 (human) | Apoptosis suppressed | [101] |
| NIH3T3 (mouse) | Fibroblasts, transient assay | [78] |
| RCL (rat) | Chloroleukemia | [90] |
| SW480 (human) | p53 defective | [51] |
| T47D (human) | Carcinoma | [52] |
| V3, XR-1 CHO (hamster) | Defective in telomeres and non-homologous end joining or DNA repair | [29] |
| YZ-5 (human) | Competent for altered telomere maintenance | [53] |