Binding immunoglobulin protein-treated peripheral blood monocyte-derived dendritic cells are refractory to maturation and induce regulatory T-cell development

Valerie M Corrigall; Olivier Vittecoq; Gabriel S Panayi

doi:10.1111/j.1365-2567.2009.03103.x

Figure 1

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Binding immunoglobulin protein (BiP) alters expression of indolamine-2,3 dioxygenase (IDO), costimulatory molecules and regulatory molecules by dendritic cells (DCs). Peripheral blood monocytes were cultured with granulocyte–macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4 in the presence or absence of BiP (20 μg/ml) for 7 days. Lipopolysaccharide (LPS; 500 ng/ml) was added for the final 48 hr to mature dendritic cells (mDCs). (a) Flow cytometry was used to detect intracellular expression of IDO after DC maturation in nine different experiments. (b) The expression of CD14 and CD86 on mDCs or mDC_(BiP)s in a representative experiment and (c) the expression of leucocyte immunoglobulin-like receptor subfamily B member 1 (LILRB1) by mDCs and mDC_(BiP)s in eight different experiments.