Fig. 2.
Elastase/LPS treatment induced goblet cell metaplasia. Paraffin lung sections of mice were stained with periodic acid-Schiff (PAS) reagent. PBS-treated mice did not show an increase in PAS-positive cells in either large or small airways (A and B). Elastase (C and D) or LPS-treated mice show few PAS-positive cells (arrows) in both large (C and E) and small airways (D and F). Elastase/LPS-treated mice show abundant PAS-positive cells (arrows) in large (G) and small airways (H), respectively. Images shown are typical of 4–6 independent experiments.