FIGURE 4.

NOS protein expression (A) and NOS activity (B) in gastrocnemius muscle of 16-week-old chow-fed C57BL/6J mice expressing a WT or kinase-dead (KD) form of AMP-activated protein kinase α2 in cardiac and skeletal muscle. A, SDS-PAGE was performed on 75 μg of whole cell lysate from gastrocnemius muscle. B, NOS activity at rest and in response to exercise was determined in gastrocnemius muscle as described (see “Experimental Procedures”). Data are mean ± S.E. for n = 5 per group. *, p < 0.05 versus corresponding WT; **, p < 0.05 versus WT_70%; †, p < 0.05 versus corresponding sedentary group.