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Neurobiol Dis. Author manuscript; available in PMC 2009 Dec 7.
Published in final edited form as:
Neurobiol Dis. 2007 Jul; 27(1): 77–89.
Published online 2007 Apr 27. doi: 10.1016/j.nbd.2007.04.005
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Mecp2 KO mice have altered presynaptic protein expression pattern

(A-C) Digitized bright-field micrographs show synaptophysin immunoreactivity in the adult and young mice. Scale bar=100 μm. (A) The box indicates the region that is enlarged in C and D. (B) Sections were incubated with normal rabbit IgG, instead of synaptophysin antibody, as a negative control. (C) Example of a brain section with small synaptophysin positive spots (arrowheads) and two large clusters (arrows). scale bar=10 μm. (D) The output of particle analysis of (C) produced by Image-J showing the two large clusters (arrows). (E) Number of large clusters in 4- and 8-week-old WT mice (**P < 0.01) compared to 4- and 8-week-old KO mice. Note that WT animals showed a clear age-dependent reduction in the density of large synapse clusters, whereas KO failed to show this developmental change.

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