Figure 3

PANEL A: Results of saturation binding and western analysis performed on COS-1 membrane preparations containing R212C and wild type constructs. PANEL B: Corresponding confocal microscopy overlay images (63 x resolution) showing predominant membrane trafficking only for wild-type protein. The hIP receptors both wild type and mutants are labeled red (1D4 monoclonal antibody). The endoplasmic reticulum are labeled green (anti-calnexin antibody) and the overlay picture additionally has blue nuclear staining (DAPI) and a phase contrast microscopic image of the cell to localize the cells perimeter. White arrows are used to localize areas of cell surface membrane.