Fig. 3.

An external file that holds a picture, illustration, etc. Object name is zpq9990907850003.jpg

Increased DNA methylation in yeast depleted of histone H3 lysine K4 methylation. (A) Western analysis for H3K4 mono-, di-, and trimethylation in strains of set1Δ, swd1Δ, swd3Δ, and spp1Δ. Blotting with antibodies against unmodified H3 and actin served as loading controls. (B) Methylcytosine contents in various strains expressing Dnmt3a alone or together with Dnmt3L. (C) Western analysis for H3K4 mono-, di-, and trimethylation in set1Δ strains expressing reintroduced WT and mutant Set1 protein. (D) Methylcytosine contents in set1Δ strains expressing reintroduced wild-type and mutant Set1 protein.

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