The N-terminus of histone H3 is required for de novo DNA methylation in chromatin

Jia-Lei Hu; Bo O. Zhou; Run-Rui Zhang; Kang-Ling Zhang; Jin-Qiu Zhou; Guo-Liang Xu

doi:10.1073/pnas.0905767106

Fig. 4.

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Distribution of DNA methylation at genomic sequences associated with unmethylated H3K4. (A) Schematic diagrams of six yeast genomic loci chosen for DNA methylation analysis. Regions for chromatin-immunoprecipitation (ChIP) assay and bisulfite-based DNA methylation analysis are indicated. (B) ChIP with anti-H3K4 trimethylation antibody to examine the histone methylation state in the regions chosen for analysis. (C) COBRA DNA methylation analysis of six representative loci in wild-type and set1Δ strains. TaqI digestion of a PCR fragment into smaller fragments reflects full or partial methylation (me) in the region analyzed. Resistance to the digestion represents an unmethylated (un) state. (D) Bisulfite sequencing profiles of DNA methylation of four representative loci. CpG sequences are shown with filled (methylated) and open (unmethylated) circles.