Figure 1.

Progress of Disease Development in bos1 Plants Inoculated with Necrotrophic Pathogens.

(A) and (B) Wild-type (left) and bos1 (right) homozygous plants at 5 (A) and 10 (B) days after inoculation with Botrytis. Plants were inoculated by spraying spore suspension at a density of 10⁵ spores/mL and kept under high humidity.

(C) Leaves from wild-type (Wt) and bos1 homozygous plants at 5 days after inoculation with a 4-μL droplet of A. brassicicola spores (10⁵ spores/mL).

(D) Lesion size was measured at 3 days after inoculation. Data points represent average lesion size ± se of measurements from a minimum of 40 lesions. This experiment was repeated three times with similar results. WT, wild type.

(E) Accumulation of the Botrytis (B.c) β-tubulin mRNA in inoculated plants. Twenty micrograms of total RNA extracted from inoculated plants was loaded per lane. dpi, days after inoculation.