Activation of the Unfolded Protein Response Contributes toward the Antitumor Activity of Vorinostat

Soumen Kahali; Bhaswati Sarcar; Bin Fang; Eli S Williams; John M Koomen; Philip J Tofilon; Prakash Chinnaiyan

doi:10.1593/neo.91422

Figure 1

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HDAC inhibition with vorinostat leads to GRP78 acetylation and dissociation with its client protein PERK. The glioblastoma cell line U251 was exposed to vorinostat (1 µM) for 6 and 24 hours. Cell lysates were immunoprecipitated (IP) for GRP78 and immunoblot (IB) was performed for (A) acetyl-lysine and (B) PERK. IB forGRP78 served as loading control.