ATRX interacts with H3.3 in maintaining telomere structural integrity in pluripotent embryonic stem cells

Lee H. Wong; James D. McGhie; Marcus Sim; Melissa A. Anderson; Soyeon Ahn; Ross D. Hannan; Amee J. George; Kylie A. Morgan; Jeffrey R. Mann; K.H. Andy Choo

doi:10.1101/gr.101477.109

Figure 4.

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Cellular localization of MYC-H3.3 and ATRX in differentiated ES cells. (A,B) MYC-H3.3 construct was transfected into ES129.1 cells and induced with 1 μM doxycycline for 24 h MYC-H3.3 (Aii, red) colocalized with TERF1 (Aiii, green) at the telomeres (examples shown by the arrows) in undifferentiated (0 day) interphase ES 129.1 cells. MYC-H3.3 and ATRX signals remained apparent at the telomeres after 6 and 9 d of induction (data not shown). However, no enrichment of MYC-H3.3 (Bii, red) was detected at the telomeres (indicated by TERF1 staining; Biii, green) 12 d following the induction of differentiation. (C,D) Likewise, ATRX association at the telomeres was also not noticeably affected by the induction of differentiation for 6 and 9 d (Ciii, green), but ATRX (Diii, green) completely delocalized from the telomeres after 12 d of induction of cellular differentiation.