Figure 5.

Colocalization of ATRX and MYC-H3.3. (A) ATRX (iii, green) clearly colocalized with MYC-H3.3 (ii, red) in undifferentiated (0 day) interphase ES129.1 cells. (B) The colocalization of ATRX (iii, green) with MYC-H3.3 (ii, red) remained prominent after 6 d of differentiation. (C) However, after 12 d of differentiation, the colocalization of ATRX (iii, green) with MYC-H3.3 (ii, red) was greatly reduced. Occasionally, strong ATRX signals were detected in the 6- and 12-d cells, but these signals were not colocalized with the telomeres (Biii,Ciii, green; arrowheads).