FIGURE 6.

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Dosage-dependent increase of surface expression of GRP78. 293T cells were transfected with the indicated amount of F-GRP78 expression plasmid. F-GRP78 and CNX were detected on the cell surface by Western blot, whereas calreticulin (CRT) was barely detected on the cell surface. β-Actin served as loading control for the input lysate, which represents 10% of the amount used for avidin pull-down to detect surface protein.

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