Synchronous Hyperactivity and Intercellular Calcium Waves in Astrocytes in Alzheimer Mice

Kishore V. Kuchibhotla; Carli R. Lattarulo; Bradley T. Hyman; Brian J. Bacskai

doi:10.1126/science.1169096

Figure 4

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Intercellular calcium waves in mice with cortical plaques

A,B) A time-lapse ΔF/F image filmstrip and data of calcium activity in an APP/PS1 transgenic mouse. The white arrow in the second panel points to the “initiator” astrocyte and the sequential panels show the propagation of the wave. C) A summary of (A) in which color denotes temporal sequence and arrows denote spatial propagation. D) Astrocytes that “initiate” ICWs were closer to senile plaques than the average active or inactive astrocyte (*, p < 0.05, Kruskal-Wallis with Tukey-Cramer post-hoc). E) There was a significant increase in the amplitude of the calcium signal during a wave (* = p < 0.001, ANOVA with Tukey- Cramer post-hoc, n = 47 cells in Tg (wave), n = 156 cells in Tg (non-wave), n = 168 in Wt).