Fig. 5.

Analysis of the interaction of DDX3 with core mutants. (a) HuH-7 cells were electroporated with different viral RNAs and HCV core co-immunoprecipitated at 72 h post-incubation using the anti-DDX3 serum R648 as described in the legend to Fig. 2b. (b) HuH-7 cells electroporated with JFH1_Y35A were fixed at 72 h post-incubation and analysed by confocal microscopy for the intracellular distribution of core, DDX3 and ADRP using appropriate antibodies. Bar, 10 μm.