The Meiotic Recombination Checkpoint Suppresses NHK-1 Kinase to Prevent Reorganisation of the Oocyte Nucleus in Drosophila

Oscar M. Lancaster; Manuel Breuer; C. Fiona Cullen; Takashi Ito; Hiroyuki Ohkura

doi:10.1371/journal.pgen.1001179

Figure 5

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Disassembly of the synaptonemal complex and loading of the condensin complex is delayed by meiotic checkpoint activation.

(A) Synaptonemal complex in wild-type and spn mutant oocytes. Ovaries were immunostained for the tranverse filament protein C(3)G and DNA. Bar = 10 µm. (B) The C(3)G staining pattern was classified as filamentous (arrowheads in A), fragmented or diffused (arrow in A). spn mutants significantly delayed disassembly of the synaptonemal complex (p<0.01; marked with asterisks). A minimum of thirteen oocytes were counted. (C) Condensin in wild-type and spn mutant oocytes. Ovaries were immunostained for the condensin subunit CAP-D2 and DNA. Bar = 10 µm. (D) Chromosome accumulation of CAP-D2 staining was classified into clear (arrow in C), weak or absent (arrowheads in C). spn mutants significantly delayed condensin loading (p<0.01; marked with asterisks). A minimum of nine oocytes were counted.