A Functional Link between the Histone Demethylase PHF8 and the Transcription Factor ZNF711 in X-Linked Mental Retardation

Daniela Kleine-Kohlbrecher; Jesper Christensen; Julien Vandamme; Iratxe Abarrategui; Mads Bak; Niels Tommerup; Xiaobing Shi; Or Gozani; Juri Rappsilber; Anna Elisabetta Salcini; Kristian Helin

doi:10.1016/j.molcel.2010.03.002

Figure 5

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PHF8 Colocalizes with ZNF711 on Target Genes

(A) Venn diagram showing the overlap and number of genes positive for ZNF711, PHF8, and H3K4me3 in human neuroblastoma SH-SY5Y cells.

(B) Binding profiles of the ZNF711 target genes JARID1C, C2ORF34, and PCBP2 for ZNF711 and PHF8 obtained from ChIP-seq analysis; the chromosomal locations are indicated in the top of the panel according to hg18. The Y axes indicate the number of sequenced reads.

(C) qChIP analysis of ZNF711 target genes JARID1C, C2ORF34, and PCBP2 in SH-SY5Y cells using antibodies against ZNF711, PHF8, H3K4me3, H3K9me2, and IgG control. The enrichment of the ChIP assays is shown as percentage bound of input.

(D) qChIP analysis of ZNF711 target genes JARID1C and C2ORF34 in HEK293 cells after shRNA-mediated ZNF711 knockdown using antibodies against ZNF711, PHF8, H3K4me3, and IgG control.

(E) Expression analysis of ZNF711-PHF8 target genes after shRNA-mediated ZNF711 and PHF8 knockdown in HEK293 cells using real-time RT-qPCR.

Error bars represent SD; n = 3.