Figure 2

(A, B) Growth curves of male (A) and female (B) mice fed a normal chow diet. Sixteen Rcan2 ^+/+, 29 Rcan2 ^+/−, and 15 Rcan2 ^−/− males were analyzed from postnatal week 3 to week 10, and 9, 16, and 7, respectively, after postnatal week 10. Eighteen Rcan2 ^+/+, 34 Rcan2 ^+/−, and 23 Rcan2 ^−/− females were analyzed from postnatal week 3 to week 10, and 11, 19, and 17, respectively, after postnatal week 10. **: P<0.001, ***: P<0.0001 (vs Rcan2 ^+/+); +: P<0.05, ++: P<0.005 (vs Rcan2 ^+/−). (C) Representative examples of 9-month-old Rcan2 ^+/+ and Rcan2 ^−/− males. (D) Mean body weights of ten 9-month-old Rcan2 ^+/+ and Rcan2 ^−/− males. (E) Mean tibia lengths of eight 9-month-old Rcan2 ^+/+ and Rcan2 ^−/− males. (F) Mean weight of epididymal and retroperitoneal white adipose tissue (EWAT and RWAT, respectively) in ten 9-month-old Rcan2 ^+/+ and Rcan2 ^−/− males. All values are given as mean ± s.e.m. ***: P<0.0001. Filled circles/columns indicate Rcan2 ^+/+ mice; open circles/columns, Rcan2 ^−/− mice.