The Combined Functions of Proapoptotic Bcl-2 Family Members Bak and Bax Are Essential for Normal Development of Multiple Tissues

Tullia Lindsten; Andrea J. Ross; Ayala King; Wei-Xing Zong; Jeffrey C. Rathmell; Helena A. Shiels; Eugen Ulrich; Katrina G. Waymire; Patryce Mahar; Kenneth Frauwirth; Yifeng Chen; Michael Wei; Vicki M. Eng; David M. Adelman; M. Celeste Simon; Averil Ma; Jeffrey A. Golden; Gerard Evan; Stanley J. Korsmeyer; Grant R. MacGregor; Craig B. Thompson

doi:10.1016/s1097-2765(00)00136-2

Figure 5

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Peripheral Lymphocytes that Accumulate in bax^–/–bak^–/– Mice Are Enriched in Memory Cells and Fail to Die by Neglect Peripheral lymphocytes from lymph node cells were analyzed by flow cytometry

(A and B) CD4 and CD8 expression (A) and Thy1.2 and B220 expression (B). Gated T cells were also analyzed for the expression of CD44 and CD62L. Gated B cells were also analyzed for expression of IgD.

(C) Peripheral lymphocytes from spleens of bax^–/–bak^–/– (open symbols) and control mice (closed symbols) were cultured for 4 days. Cells were stained with PI and subjected to FACS analysis at the indicated times. Samples were assayed in triplicate.