TABLE 1
Intraphagosomal peroxynitrite controls wild type T. cruzi infection
| Macrophage treatment | T. cruzi amastigotes/100 macrophagesa | ||
|---|---|---|---|
| Wild type | TcCPX | ||
| Unstimulated | 37 ± 13 | 38 ± 19 | |
| +Apocynin | 38 ± 12 | NDb | |
| IFN-γ/LPS-prestimulatedc | 14 ± 3* | 36 ± 9 | |
| +Apocynin | 33 ± 14 | NDb | |
| IFN-γ/LPS post-infectionc | 27 ± 8 | NDb | |
a The load of T. cruzi amastigotes in the macrophage culture was evaluated at 24 h after infection with metacyclic trypomastigotes (parasite:macrophage ratio, 5:1). Data are mean ± S.E. of three independent experiments.
b Not determined.
c IFN-γ/LPS was added to the macrophage culture 5 h before infection (prestimulated) or 30 min after infection (post-infection). Apocynin (50 μm) was used when indicated to avoid NADPH oxidase assembly.
*, p < 0.01.