Figure 3.

EGCG antagonizes AR-positive PCa cell growth. A, B) C4-2 (A) and 22Rν1 cells (B) were treated with indicated concentrations of EGCG for 48 h, and cell viability was determined by MTT assay, as detailed in Materials and Methods. *^aP < 0.01 vs. control group; *^bP < 0.01vs. 20 μM EGCG group; 1-way ANOVA followed by Tukey's HSD test. C) EGCG modulated the expression of miRNAs considered to be important in PCa. Relative expression of miRNA-21 and -330 in tumor xenograft tissues isolated from animals treated with EGCG. Real-time PCR was performed for the 2 miRNAs, and fold change was calculated.