Fig. 1.

GP images of living macrophages, GP distribution, and temperature dependency. 3D-reconstructed pseudocolored GP images of RAW264.7 cell (image plane parallel to coverslip, viewed from above) (A) and THP-1 cell (viewed from below) (B). Circled area (arrow) 126 pixels/0.73 μm²; widths are 28.3 μm(A) and 39.0 μm(B). (C) Distribution of a stack of GP images (⋄) was deconvoluted by fitting two Gaussian distributions to the experimental data (black line) with centers of GP = 0.140 (center C1) and GP = 0.549 (center C2). Area under each curve equates to surface coverage. (D) Temperature dependence of the GP centers (□, C1, slope –0.0112; ⋄, C2 slope –0.0132; left axis) and coverage of center C2 as percent of all Laurdan-stained pixels (Δ slope –3.566, right axis). Solid symbols are the means of 10–12 stacks (Table 1); open symbols are data from single stack.