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Fig. 2.

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PERK inhibition partially reverts suspension-induced autophagy. (A) Lysates from adhered (A) or 6 h suspended (S) β-Gal and PERKΔC MCF10A cells were analyzed by IB for the indicated antigens. Densitometric analysis for LC3 flux was done using Image J software (n = 3). (B) Lysates of PERK+/+ and PERK−/− mouse embryonic fibroblasts (MEFs) were collected at the indicated time points, and the indicated antigens were detected by IB. (C) MCF-10A lysates from attached (A) and suspended (S) cells that were transfected with ATG7, PERK, or control (C) siRNA were used for IB against the indicated antigens. Results for the ATG7 and PERK knockdown controls are shown in the right panel.

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