Figure 5

Distinct effects of mutations in SAGA and TFIID components on gene cohorts affected by Mediator tail deletions. (A, B) The fraction of all members in a compendium of chromatin modifier mutants (Steinfeld et al, 2007) having a specific ΔExpression value is plotted as a function of ΔExpression, where the latter is equal to the difference between the average change in expression of the indicated cohort (genes showing decreased or increased expression in gal11Δ med3Δ yeast) and the average change in expression of all genes for each mutant in the compendium. The vertical lines indicate one standard deviation above and below the mean ΔExpression for each plot. (C) Venn diagram showing interrelationships between genes showing decreased expression in gal11Δ med3Δ yeast, and TFIID-dominated and SAGA-dominated genes (Huisinga and Pugh, 2004). (D–F) Plots showing relative frequency of genes having indicated changes in expression in spt3, taf1, and H3 K5, 9, 14, 18, 23, 27Q mutants for all genes (solid lines) and for the cohort of genes showing decreased (D, E) or increased (F) expression (dashed lines) in gal11Δ med3Δ yeast. Calculation of the Kolmogorov–Smirnov (K–S) statistic, which is useful for assessing disparities between non-normal distributions, for the distributions in (D–F) yielded P-values <10⁻¹⁵ in all three cases.