Figure 5
DLBCL-associated mutations in the CREBBP HAT domain decrease its affinity for Acetyl-CoA
a, Western blot analysis of in vitro acetyltransferase reactions performed using the wild-type or mutant Y1450C CREBBP-HA recombinant protein and a GST-p53 substrate in the presence of decreasing amounts of Acetyl-CoA. Anti-HA and anti-p53 antibodies document the presence of equivalent amounts of effector and substrate proteins in the reaction. b, In vitro acetyltransferase activity of the indicated CREBBP-HA mutant proteins in the same assay using 25nM Acetyl-CoA.