Permissive Roles of Phosphatidyl Inositol 3-Kinase and Akt in Skeletal Myocyte Maturation

Elizabeth M. Wilson; Jolana Tureckova; Peter Rotwein

doi:10.1091/mbc.E03-05-0351

Figure 10.

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Akt restores myocyte maturation after inhibition by LY294002. Results are shown of C2BP5 cells infected with Ad:MyoD or with both Ad:MyoD and Ad: iAkt. Cells were incubated in DM ± LY294002 (LY, 20 μM) and ± HT (1 μM) for 1 or 2 d. (A) Results of immunocytochemistry for MHC (red) and myogenin (green) after 2 d in DM. Magnification, 200×. (B) Quantification of myotube area by using MHC staining (mean ± SEM of three experiments counting a total of 12 fields at 200× magnification). (C) Immunoblots for myogenin, MHC, MEF2C, MEF2D, Akt, and phospho-Akt (Ser473) (p-Akt) by using whole cell protein lysates.